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Fomin P.D.1, Sydorchuk R.I.2, Olsson S.Å.3, Sydorchuk I.I.2, Sydorchuk L.P.2, Staёl von Holstein C.4 National State Medical University of Ukraine1, Bucovinian State Medical Academy (Ukraine)2, Hospital Ängelholm-Helsingborg (Sweden)3, University of Lund (Sweden)4 Background. Due to immune disorders playing a key role in development of Systemic Inflammatory Response Syndrome (SIRS) passive immune therapy is considered to be a method of choice for abdominal sepsis (AS) patients. Existing remedies (specific hyper immune serum, specific antibodies and immunoglobulins) are expensive and require exact validation of pathogens. The aim of the study was to evaluate the efficacy of using the AS reconvalescent donors plasma for passive immunotherapy of AS. Method. The study was conducted either experimentally on 775 Wistar line rats and 38 inbreed dogs. Totally 296 patients with AS were also involved into the study; 58 formed control group; 26 patients were selected as reconvalescent donors of plasma. Serum concentrations (ELISA) of major antibodies were determined against most significant pathogens (E.coli, Staphylococcus spp., S.aureus, Bacteroides spp, K. pneumoniae, P. aeruginosa). Results. Changes of serum antibodies concentrations were time dependent and fluctuating during the current of AS forming the waveform curve. Most remarkable decreases were found during 24-72 hours of AS. Serum antibodies titres to main pathogens were slightly higher due to antibiotics and detoxication therapy. Operation by itself decreased titres from 4.42±0.28 to 3.49±0.25 (E.coli), from 5.41±1.02 to 3.0±0.58 (P.aeruginosa). Antistaphylococcal antibodies titres decreased from 7.22±0.9 before surgery to 4.83±0.47 after. Repeated operations alter antibodies concentrations even more significantly. The highest levels of antibodies were found in patients who underwent successful treatment of AS one to two months prior to investigation. Their plasma was used in treatment of AS patients. Intravenous administration of two-dose 100-200 ml of hyper immune plasma per day prevented following decrease of antibodies levels and in 98% case increased them (21.39±1.47%). The cost of treatment was 15-37% lower if compare with traditional methods (control group). Conclusion. There is an exact evidence of hyperimmune plasma using efficacy in patients with abdominal sepsis; it is more cost effective if compare with traditional methods of immunotherapy.